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Immortalized Rat Mesencephalic (AF5) Cells-SV40T (T155g)
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Cat.No.
CSC-I9310L
Description
Immortalized Rat Mesencephalic (AF5) Cells - SV40T are derived from E-14 rat embryos. Immortalization was preformed with the truncated SV40 large T lacking the p53 binding domain gene via transfection. AF5 cells express glial and neuronal markers, indicative of mature phenotypic properties. It is a reliable source for central nervous system-related studies.
Source
Mesencephalic tissue
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Immortalized via transfection with pCMV/SVE/neo carrying the N-terminal fragment of SV40 large T (T155g)
Markers
Fibronectin, vimentin, MAP-2, and Thy-1
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) 500 µg/ml G418 used for selection
2) Western blot to confirm presence of SV40 and markers
2) Western blot to confirm presence of SV40 and markers
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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