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Immortalized Rat Conjunctival Epithelial Cells (CJ4.3)-SV40
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Cat.No.
CSC-I9201L
Description
The Immortalized Rat Conjunctival Epithelial Cells (CJ4.3) were derived from primary conjunctival epithelial cells from Fischer 344 rats. The primary cells were immortalized with pSV(neo) and transfected with SV40 large T antigen. The immortalized cells display epithelial morphology and express the conjunctiva-specific cytokeratin 4, the goblet cell-specific cytokeratin 7 and were negative for the corneal epithelial cell-specific cytokeratin 12. The Immortalized Rat Conjunctival Epithelial Cells (CJ4.3) is useful as models for studying the conjunctival epithelial barrier and also in developing strategies for delivering macromolecular antigens across this barrier to underlying components of the immune system.
Source
Eye
Culture Properties
Adherent
Morphology
Epithelial
Immortalization Method
Immortalization by transfection with SV40 large T antigen
Markers
Cytokeratin 4, Cytokeratin 7
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Quality Control
1) Immunofluorescence confocal microscopy demonstrating cytokeratin expression
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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