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Immortalized Rat Arachnoid Cells-SV40/hTERT
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Cat.No.
CSC-I9080L
Description
The arachnoid cells are important in the maintenance of apical-basal polarity and the formation of tight junctions at the cerebrospinal fluid-blood barrier (CSF-blood barrier). The Immortalized Rat Arachnoid cells-SV40/hTERT derived from primary rat arachnoid cells maintained the ability to form intact epithelial monolayer and displayed normal immunohistochemical and morphological phenotypes. As such, the Immortalized Rat Arachnoid cell line is useful in physiological studies in modeling arachnoid granulations and cerebrospinal fluid flow. In addition, this cell line is also useful in pathological conditions such as hydrocephalus, arachnoid cysts, or other disorders.
Source
Brain
Culture Properties
Adherent
Morphology
Spindle-like appearance at low density, cobblestone appearance at confluency
Immortalization Method
Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen and hTERT gene
Markers
vimentin, cytokeratin and desmoplakin
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1. Immunofluorescence and Western Blotting were used to confirm the presence of SV40 (82kDa) and hTERT (122kDa) expression.
2. Immortalized Rat Arachnoid Cells exhibit spindle-like appearance at low density before reaching confluency and “cobblestone” appearance after becoming fully confluent, which appeared to be identical to its primary counterparts.
3. Immunofluorescence and Western Blotting were used to confirm the presence of arachnoid cell markers such as vimentin, cytokeratin and desmoplakin.
2. Immortalized Rat Arachnoid Cells exhibit spindle-like appearance at low density before reaching confluency and “cobblestone” appearance after becoming fully confluent, which appeared to be identical to its primary counterparts.
3. Immunofluorescence and Western Blotting were used to confirm the presence of arachnoid cell markers such as vimentin, cytokeratin and desmoplakin.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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