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Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC-GFP)
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Cat.No.
CSC-I9283L
Description
Immortalized Mouse Subcutaneous Adipose Multipotent Mesenchymal Cells (m17.ASC-GFP) were genetically modified by lentiviral vectors carrying green fluorescent protein
(GFP). When injected in the spleen of NOD/SCID-gnull monocrotaline-treated mice, m17.ASC-GFP cells efficiently engrafted in the liver, candidating this cell line as a valuable stem cell model.It stably expresses the stem cell markers Sca-1, c-Kit/CD117, nestin, nucleostemin, CD44, and CD106, but is negative for the embryo stem cell markers Nanog, Oct4, and Sox2. The cells were endowed with multipotency and can be induced to differentiate toward osteogenic, chondrogenic, adipogenic, and cardiogenic phenotypes. It displays a normal karyotype and stable telomeres, neither proliferates when plated in soft agar nor gives rise to tumors when injected subcutaneously in NOD/SCID-g null mice. The cell line is thus recommended as a valuable tool for a number of applications inex vivotissue engineering to study the differentiation mechanisms involved in tissue repair, as well as a model for both pharmacological and toxicological studies.
(GFP). When injected in the spleen of NOD/SCID-gnull monocrotaline-treated mice, m17.ASC-GFP cells efficiently engrafted in the liver, candidating this cell line as a valuable stem cell model.It stably expresses the stem cell markers Sca-1, c-Kit/CD117, nestin, nucleostemin, CD44, and CD106, but is negative for the embryo stem cell markers Nanog, Oct4, and Sox2. The cells were endowed with multipotency and can be induced to differentiate toward osteogenic, chondrogenic, adipogenic, and cardiogenic phenotypes. It displays a normal karyotype and stable telomeres, neither proliferates when plated in soft agar nor gives rise to tumors when injected subcutaneously in NOD/SCID-g null mice. The cell line is thus recommended as a valuable tool for a number of applications inex vivotissue engineering to study the differentiation mechanisms involved in tissue repair, as well as a model for both pharmacological and toxicological studies.
Species
Mus musculus
Source
Adipose tissue
Culture Properties
Adherent
Morphology
Fibroblast-like
Immortalization Method
Spontaneous immortalization
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) RT-PCR and Cytofluorimetry were performed on the cDNA from the cells to confirm the expression of stem cell markers Sca-1, c-kit, Islet 1, nestin, and nucleostemin.
2) Cells after differentiation were specifically stained with alizarin red, adipo-red and Alcian blue, and the tissue-specific collagen II antibodies, respectively to confirm osteogenic, adipogenic, and chondrogenic phenotypes the cells’ multipotency.
3) G-banding analysis for macroscopic chromosome alterations over time compared with normal mouse karyotys.
4) GFP transduction was confirmed by culturing with antibiotics.
2) Cells after differentiation were specifically stained with alizarin red, adipo-red and Alcian blue, and the tissue-specific collagen II antibodies, respectively to confirm osteogenic, adipogenic, and chondrogenic phenotypes the cells’ multipotency.
3) G-banding analysis for macroscopic chromosome alterations over time compared with normal mouse karyotys.
4) GFP transduction was confirmed by culturing with antibiotics.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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