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Immortalized Mouse Smooth Muscle/Myometrial-Like Cells (SMU1-10)

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Cat.No.
CSC-I9251L
Description
During preganancy the myometrium undergoes hypertrophy and hyperplasia. The established Immortalized (Conditionally) Mouse Smooth Muscle/Myometrial-Like Cells (SMU1-10) is recommended for use as an in vitro model of smooth muscle/myometrial differention and for steroid hormone gene regulation studies. It proliferates at a permissive temperature of 33°C and exhibits smooth-muscle related markers such as the SM-MHC, SM22 alpha, and connexin 43. Thromboxane A2 receptors and progesterone receptors are also present. The SMU1-10 cells expresses smooth muscle gamma-actin (SMGA) which is a maker for smooth muscle differentiation. This cell line is recommended for studies and analysis in smooth muscle/myometrial differentiation, maturation, and the role of steroid hormones in gene regulation.
Species
Mus musculus
Source
Uterus
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Isolated from a H-2Kb-tsA58 transgenic mouse carrying a thermolabile SV40 large T-antigen gene
Markers
SM-MHC, SM22 alpha, connexin 43, thromboxane A2 receptor, progesterone receptor
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
Indirect immunofluorescence, Northern blot, and RT-PCR were utilized for smooth muscle-related markers.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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