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Immortalized Mouse Sertoli Cells (ST38c)
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Cat.No.
CSC-I9278L
Description
Previously immortalized sertoli cells- involved in androgen regulation during spermatogenesis- lose androgen receptor (AR) expression or androgen responsiveness; thus leaving them ill-suited for any further research regarding androgen regulation. The Immortalized Mouse Sertoli Cells (ST38c) eliminate this barrier with their continuous expression of endogenous AR. These cells are perfectly suited for investigating androgen receptor signaling in sertoli cell function in order to determine both molecular and cellular mechanisms.
Species
Mus musculus
Source
Testes
Culture Properties
Adherent
Morphology
Spindle-shaped
Immortalization Method
Isolated from a transgenic mouse carrying SV40 large T Antigen under the control of the human vimentin promoter
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Androgen receptor mRNA was confirmed via RT-PCR while western blot confirmed AR protein expressn.
2) Androgen receptor regulated genes were identified via microarray assays.3. SV40 Tag expression confirmed through fluorescence microscopy.
2) Androgen receptor regulated genes were identified via microarray assays.3. SV40 Tag expression confirmed through fluorescence microscopy.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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