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Immortalized Mouse Podocytes-SV40 (MPC-5)
Cat.No.: CSC-I2320Z
Species: Mouse
Culture Properties: Adherent
- Specification
- Q & A
- Customer Review
Cat.No.
CSC-I2320Z
Description
The conditionally Immortalized Mouse Podocytes-SV40 cell line allows an in-vitro process of maturation analogous to the development and maturation of podocytes in-vivo. Both proliferating
and differentiating MPC cells express the WT-1 protein and an ordered array of actin fibres and microtubules extends into the forming cellular processes during differentiation, reminiscent of
podocyte processes in vivo. The result is a homogenous, stable cell source that shows expression of key antigenic markers of differentiated in-vivo podocytes. These include the novel podocyte
proteins nephrin, podocin, CD2AP and synaptopodin.
Immortalization is conditional by use of a temperature-sensitive transgene encoding SV40LT.
and differentiating MPC cells express the WT-1 protein and an ordered array of actin fibres and microtubules extends into the forming cellular processes during differentiation, reminiscent of
podocyte processes in vivo. The result is a homogenous, stable cell source that shows expression of key antigenic markers of differentiated in-vivo podocytes. These include the novel podocyte
proteins nephrin, podocin, CD2AP and synaptopodin.
Immortalization is conditional by use of a temperature-sensitive transgene encoding SV40LT.
Species
Mouse
Recommended Medium
Immortalized Mouse Podocyte Medium (CM-I2320Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
a temperature-sensitive transgene encoding SV40LT
Shipping
Dry Ice
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20°C.
Note: Never can cells be kept at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here
to submit the PubMed ID of your paper to get a coupon.
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