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Immortalized Mouse OP9 Stromal Cells (I-OP9)
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Cat.No.
CSC-I9271L
Description
The Immortalized Mouse OP9 Stromal Cells (I-OP9) is a reliable source of M-CSF-deficient mouse stromal cells which has been reported to be able to support the differentiation of pluripotent stem cells (iPSCs), such as hematopoietic stem cells (HSC). The OP9 cells were immortalized through viral infection with retrovirus carrying the LXSN16E6E7 vector to generate I-OP9. I-OP9 cells are tumorigenic when injected into mice. However, treatment with mitomycin C beforein vitrodifferentiation will eliminate the tumorigenicity issue before using as feeder cells for HSC. Additionally, the Immortalized Mouse OP9 Stromal Cells (I-OP9) may be used to study functions of M-CSF deficiency and osteroporosis.
Species
Mus musculus
Source
Calvaria
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Serial passaging and infection with retrovirus carrying LXSN16E6E7
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Cells are tested to propagate over 42 passages, and retained phenotypes similar to the primary cells;
2) HPV gene was detected via RT-PCR.
2) HPV gene was detected via RT-PCR.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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