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Immortalized Mouse Neuronal Cells (M4b)
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Cat.No.
CSC-I9345L
Description
The Immortalized Mouse Neuronal Cells (M4b) were derived from the spinal cord of a fetal mouse after 12-16 days of gestation. The fetal mouse was bred from double heterozygous (Rb 2H/RB 32 Lub) males and normal C57BL females. Transformation of the cell line was induced via supplementation with UCHT1 rat thyroid cell line-conditioned medium and was confirmed via the detection of vimentin. Immunohistochemistry found the presence of neurological makers, but the absence of glial markers in the Immortalized Mouse Neuronal Cells (M4b). In addition, the intracellular [Ca2+] response after neurotransmitter stimulation was rapid and transient as expected. The Immortalized Moue Neuronal Cells (M4b) is useful in studies of neuronal cell biology, viral infections, and neuronal cytotoxicity. The Immortalized Moue Neuronal Cells (M4b) can also be used as a control for the Immortalized Trisomy 16 Mouse Neuronal Cells (MTh), which is a mouse model of Down Syndrome.
Species
Mus musculus
Source
Spinal Cord
Culture Properties
Adherent
Morphology
Neuronal
Immortalization Method
Supplementation of plating medium with UCHT1 rat thyroid cell line-conditioned medium
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) RT-PCR for SOD-1 and GAPDH;
2) Immunohistochemistry of neuronal markers;
3) Intracellular Ca2+measurements.
2) Immunohistochemistry of neuronal markers;
3) Intracellular Ca2+measurements.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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