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Hot Products

Immortalized Mouse Lung Microvascular Endothelial Cells-SV40
Cat.No.: CSC-I2306Z
Species: mouse
Morphology: Polygonal
Culture Properties: Adherent
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Cat.No.
CSC-I2306Z
Description
Immortalized Mouse Lung Microvascular Endothelial Cells-SV40 have been obtained immortalizing Mouse Lung Microvascular Endothelial Cells with SV40 gene. Immortalized cells were controlled passaging side by side with the primary cells. Primary cells go into senescence after the 4th passage while the SV40 genetranduced cells go beyond 20 passages. This cell line is a great in vitro model system.
Species
mouse
Tissue of Origin
Lung
Recommended Medium
SuperCult® Immortalized Mouse Lung Microvascular Endothelial Cell Medium (Cat No.: CM-I2306Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Application
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Storage
Store in liquid nitrogen
Shipping
Ship in dry ice
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here
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