Immortalized Mouse Kidney Epithelial Cells-SV40 (TCMK-1)

The TCMK-1 cell line, derived from the renal tissue of Mus musculus and immortalized via the stable integration of the Simian Virus 40 (SV40) Large T antigen, is a gold-standard in vitro model for nephrology and epithelial cell biology. While primary renal epithelial cells are prone to rapid phenotypic dedifferentiation and replicative senescence, the TCMK-1 line provides a robust, continuous system that faithfully recapitulates the physiological complexities of the kidney's tubular architecture.

• Robust Proliferative Capacity: By bypassing the p53 and pRb-mediated cell cycle checkpoints, TCMK-1 cells offer an indefinite replicative lifespan. This ensures an inexhaustible cell source, providing the high-level reproducibility and lot-to-lot consistency required for large-scale, longitudinal studies.
• Maintenance of Epithelial Fidelity: These cells retain essential epithelial characteristics, including a characteristic cobblestone morphology, the formation of polarized monolayers, and the expression of kidney-specific markers. This makes them a high-fidelity surrogate for investigating renal transport mechanisms and barrier function.
• Validated Model for Nephrotoxicity: TCMK-1 is widely utilized as a screening platform for Drug-Induced Kidney Injury (DIKI). Its standardized growth kinetics and metabolic profile allow for precise evaluation of nephrotoxic compounds, bridging the gap between early-stage discovery and in vivo preclinical validation.
• Versatile Research Applications: Beyond toxicology, these cells serve as a premier model for studying renal fibrogenesis, electrolyte homeostasis, and the cellular response to acute kidney injury (AKI) or chronic kidney disease (CKD) pathways.

Designed for the rigorous requirements of biopharmaceutical R&D, our Immortalized Mouse Kidney Epithelial Cells (TCMK-1) eliminate the variability and technical challenges associated with primary cell isolation. By providing a stable and biologically relevant "assay-ready" system, this product is an indispensable asset for researchers aiming to accelerate the development of novel renal therapeutics and diagnostic strategies.