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Immortalized Mouse Hepatocyte SIMH (CLOCK) Cells
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Cat.No.
CSC-I9332L
Description
The Immortalized Mouse Hepatocyte SIMHCLOCKCells contain a bioluminescent marker gene that reports on the circadian phase of the cells. The presence of the Per2:Luc reporter gene allows real time monitoring of clock phase. The major characteristics of this cell line include its high metabolic capacity, liver-like properties, robust synchronizable circadian clock, and capacity to stably express additional marker. When compared with other established cell lines (i.e. HepG2), the SIMHCLOCKcells show a significantly better metabolic and xenobiotic capacity. This ability of the Immortalized Mouse Hepatocyte SIMHCLOCKCells is useful in studying treatment of liver cells with various physical or chemical compounds at defined stages of the circadian cycle and analyze the time-of-day exposure effects.
Species
Mus musculus
Source
Liver
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Transfection with mammalian expression vector carrying SV40 gene
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-C2125 Mouse Hepatocytes
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Bioluminescence recording of luciferase activity;
2) Apoptosis assay using exposure to B[a]P;
3) qPCR;
4) RNA Seq analysis
2) Apoptosis assay using exposure to B[a]P;
3) qPCR;
4) RNA Seq analysis
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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