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Hot Products

Immortalized Mouse Hepatic Sinusoidal Endothelial Cells-SV40
Cat.No.: CSC-I9077L
Species: Mus musculus
Source: Liver
Morphology: Cobblestone-like
Culture Properties: Adherent
- Specification
- Q & A
- Customer Review
Cat.No.
CSC-I9077L
Description
Hepatic sinusoidal endothelial cells (HSECs) are an unique sub-population of liver that lines the hepatic sinusoids. Their role involves in clearing waste products from the blood, regulating pericyte contractility and innate immune functions. The immortalized Mouse Hepatic Sinusoidal Endothelial Cells- SV40 is derived from murine HSECs that have maintained endothelial characteristics and HSEC-specific features such as vWF and caveolin-1. These cells also migrate in response to angiogenici growth factors, form vascular tube-like structures, endycytose acetylated low-density lipoprotein (AcLDL) and secrete proteins involved in matrix remodelling. In addition, these cells are easily transduced by retrovirus. The Immortalized Mouse Hepatic Sinusoidal Endothelial Cells- SV40 is therefore suitable for studying liver endothelial cell biology, including motility and angiogenesis.
Species
Mus musculus
Source
Liver
Culture Properties
Adherent
Morphology
Cobblestone-like
Immortalization Method
Serial passaging and transduction with pantropic recombinant retroviruses carrying SV40 Large T antigen
Markers
vWF and caveolin-1
Application
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1. Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
2. Western blot was used to confirm the presence of endothelial markers such as vWF and caveolin-1.
2. Western blot was used to confirm the presence of endothelial markers such as vWF and caveolin-1.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here
to submit the PubMed ID of your paper to get a coupon.
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