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Immortalized Mouse EGFR Mutant M4
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Cat.No.
CSC-I9349L
Description
The Immortalized Mouse EGFR Mutant M4-/-Epidermal Keratinocytes were derived from primary keratinocytes of newborn EGFR null mice. The primary keratinocytes were immortalized in subsequent cultures after passaging for an extended period. The immortalized cells display similar phenotype to the primary keratinocytes including the cobblestone-shape morphology. The Immortalized Mouse EGFR Mutant M4-/-Epidermal Keratinocytes are non-tumorigenic. They also differentiate with increased calcium, maintain a relatively stable karyotype no more aberrant than cultured primary keratinocytes, and produce epidermis upon orthotopic grafting onto nude mouse hosts. Upon introduction of v-rasHaoncogene, the Immortalized Mouse EGFR Mutant M4-/-Epidermal Keratinocytes produce squamous cell carcinoma in orthotopic skin grafts. These cells are useful in studies focusing on epidermal keratinocyte biology and pathology.
Species
Mus musculus
Source
Skin
Culture Properties
Adherent
Morphology
Cobblestone-shaped
Immortalization Method
Immortalization in subsequent cultures
Markers
Keratin 14, Keratin 6
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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