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Immortalized Mouse Distal Tubule KPT2 Cells
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Cat.No.
CSC-I9296L
Description
Immortalized Mouse Proximal Tubule KPT2 Cells are spontaneously immortal derived cells from murine kidney. It does not express high levels of the Ca2+-permeable non-selective cation channel, TRPV4, unlike the Immortalized Mouse Distal Tubule KDT3 Cells. However, expresses SGLT2, an electrogenic sodiumglucose transporter-2, which may have a role in gentamicin-induced toxicity. KPT2 cells may be used to assess the effects of the presence of SGLT2 and the absence of TRPV4 has on aminoglycosides uptakein vitro.Additionally, these cells are the wild type to the Stable TRPV4 Expressing KPT2 Cell Line.
Species
Mus musculus
Source
Kidney
Culture Properties
Adherent
Morphology
Fibroblast-like
Immortalization Method
Spontaneously immortalized in culture
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
1) RT-PCR was used to detect the presence or absence of TRPV4 transcript.
2) Immunofluorescence was utilized to assess the presence of SGLT2.
2) Immunofluorescence was utilized to assess the presence of SGLT2.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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