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Immortalized Mouse CD81+/− Macrophage Cells (2BSD1.10)
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Cat.No.
CSC-I9299L
Description
Immortalized Mouse CD81−/− Macrophage Cells (ASD1) is established from the long bones of CD81 -/- mice, which were immortalized with the SV40 large T antigen and underwent limiting dilution cloning. Immortalized Mouse CD81+/− Macrophage Cells (2BSD1.10) was generated from transfecting the ASD1 cells with the pCDNA3.1/Hygro vector carrying the CD81 gene. The cell line offers users the ability to conduct experiments with a reliable source of immortal cellsin vitro. The CD81 gene has associations with malignancies as it is localized within the tumor-suppressor gene region. Hence valuable for studies in regards to cancer.
Species
Mus musculus
Source
Marrow from long bone
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Transfection preformed with pSV3-SV40-T antigen plasmid DNA
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
RT-PCR was used to detect the presence of SV40 large T antigen and CD81
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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