Immortalized Mouse Cardiac Myocyte Cell Line (HL-1)

Immortalized Mouse Cardiac Myocyte Cell Line (HL-1)

Cat.No.: CSC-I2286Z

Species: Mouse

Morphology: Polygonal

Culture Properties: Adherent

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Cat.No.
CSC-I2286Z
Description
HL-1 is an immortalized mouse atrial cardiomyocyte cell line. These cells are able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 cells can be serially passaged without losing their differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation, as well as to address pathological conditions such as hypoxia, hyperglycemia‑hyperinsulinemia, apoptosis, and ischemia‑reperfusion. The HL-1 line was derived from an AT-1 subcutaneous tumor excised from an adult female C57BL/6J mouse. The parental AT-1 line was originally derived from an atrial tumor growing in a transgenic mouse in which expression of the SV40 large T‑antigen was targeted to atrial cardiomyocytes via the atrial natriuretic factor (ANF) promoter.
Species
Mouse
Recommended Medium
Immortalized Mouse Cardiac Myocyte Cell Medium (Cat No.: CM-I2286Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
SV40 large T antigen
Application
For Research Use Only
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Quality Control
Real Time PCR was used to quantify SV40T gene expression in immortalized cell line.
free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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