Featured Products
Our Promise to You
Guaranteed product quality, expert customer support
Immortalized Mouse Articular Chondrocytes (iMAC)
- Specification
- Recommended Products
Cat.No.
CSC-I9225L
Description
The Immortalized Mouse Articular Chondrocytes are hygromycin-resistant, non-tumorigenic reversibly immortalized cells that retains both morphological and chondrogenic features. Specifically, it expresses chondrocyte markers such as Col 2a1 (type II collagen), aggrecan, Sox9, TNFα, IL-6, and COX2. In the presence of BMP2, the cells are able to produce mature chondroid matrix. These cells are unique, as the immortalization process may be reversed by infection with Cre-recombinase expressing adenoviruses, thus reverting immortal cells back into primary cells. These cells are a valuable tool in chondrogenesis molecular mechanism, and cartilage tissue regeneration studies to identify biofactors promoting proliferation and differentiation of chondrogenic progenitors.
Species
Mus musculus
Source
Tibial Plateau, Femoral Head, and Femoral Condyles
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Serial passaging and infection with retrovirus SSR#69 expressing SV40 large T antigen flanked with Cre/loxP sites
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) RT-PCR, qPCR, sqPCR analysis for mouse gene of interest;
2) Col 1al immunohistochemical staining preformed;
3) Western blot used to detect SV40 large T antigen presence;
4) Alcian blue staining analysis for chondroid matrix production.
2) Col 1al immunohistochemical staining preformed;
3) Western blot used to detect SV40 large T antigen presence;
4) Alcian blue staining analysis for chondroid matrix production.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
- Q & A
- Customer Review
Customer Support & Price Inquiry
