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Immortalized Human Skin Fibroblast-SV40
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Note: Never can cells be kept at -20 °C.
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
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Thaw frozen tubes containing 1 mL of cell suspension by rapid shaking in a 37℃ water bath, add 5 mL of culture medium and mix well. Centrifuge at 1000 rpm for 5 minutes, discard the supernatant, replenish 4-6 mL of complete medium and blow well. All cell suspensions are then added to culture flasks and incubated overnight (or cell suspensions are added to 6 cm dishes) and incubated overnight. The next day, the solution is changed and the cell density is checked.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Professional
Creative Bioarray is a professional in the field of cells and has helped me a lot.
12 Feb 2021
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