Immortalized Human Osteoblasts-SV40T+hTERT

Immortalized Human Osteoblasts-SV40T + hTERT are an osteogenic cell line that were created from primary human osteoblasts that were extracted from either cortical or trabecular bone. These cells are stably immortalized by SV40 large T antigen dual immortalization and hTERT overexpression. Morphologically, these cells retain epithelial-like adhesion in a polygonal shape, and express alkaline phosphatase (ALP), type I collagen, Runx2, osteocalcin, and osteopontin, all of which are markers expressed by osteoblasts. They are also able to retain high mineralization capacity, elevated ALP activity, and the deposition of calcium under osteogenic induction media, all of which are characteristic of osteoblasts in vivo.

Functionally, SV40T + hTERT osteoblasts are highly sensitive to bone-metabolic hormonal, inflammatory, and mechanical signals, while still retaining their ECM-producing, mineral-depositing, and RANKL/OPG signaling capabilities, and thus can be used as a model system for in vitro studies of bone remodeling. For this reason, the SV40T + hTERT osteoblast cell line can be used in many different studies, including, but not limited to the research of osteoporosis, the biocompatibility of biomaterials, osteogenic differentiation, bone regeneration, and anabolic/anti-resorptive drug screening.