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Immortalized Human Endocervical Epithelial Cells-HPV E6/E7 (A2EN)
Cat.No.: CSC-I9248L
Species: Homo sapiens
Source: Endocervix
Morphology: Cobblestone
Culture Properties: Adherent
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Note: Never can cells be kept at -20 °C.
2) Transepithelial electrical resistance was measured to measure cell monolayer functional integrity;
3) RT-PCR, Western blotting, and immunostaining were performed to compare immortalized cell characteristics to normal counterparts;
4) Expression levels of antimicrobial peptides, cytokines, and chemokines were measured to further assess immortalized cell characteristics;
5) qPCR was performed to analyze toll-like receptor protein expression between basolateral and apical components of cells, while qRT-PCR was performed to determine hormone receptor functionality.
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