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Immortalized (Conditionally) Rat Decidual Cells (GG-AD)
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Cat.No.
CSC-I9221L
Description
The decidual cells play an important role in the maintenance of pregnancy at the maternofetal interface after implantation of the fertilized egg into the endometrium. The Immortalized Rat Decidual Cells (GG-AD) is conditionally immortalized through the temperature-sensitive simian virus (SV40 tsA209) and possesses several endocrinological features, including the expression of progesterone receptor, activin type II, activin βA and activin βB. In addition, it also retains the ability to respond to cAMP stimulation and secrete PRL-related hormones, a hallmark of the antimesometrial decidual cells. This cell line is useful for studying the mechanism of decidual cell regression and reorganization.
Source
antimesometrial decidua
Culture Properties
Adherent
Morphology
Binucleated, small and compact at permissive temperature; polynucleated, differentiated and flat at non-permissive temperature.
Immortalization Method
Serial passaging and infection by SV-40 tsA209 Temperature Sensitive Mutant virus
Markers
IL-6, IL-6R, gp130, activin βA, decidual PRL-related protein (dPRP)
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
1) Northern blot performed to analyze presence of activin βA , follistatin and PLP-B mRNA;
2) RT-PCR analysis of progesterone receptor, activin βA, activin βB, and activin receptor II mRNA was completed with L19 ribosomal protein mRNA as control.
2) RT-PCR analysis of progesterone receptor, activin βA, activin βB, and activin receptor II mRNA was completed with L19 ribosomal protein mRNA as control.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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