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Hot Products

Immortalized (Conditionally) Mouse Stomach Epithelial Cells (ImSt)
Cat.No.: CSC-I9187L
Species: Mus musculus
Source: Stomach
Culture Properties: Adherent
- Specification
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Cat.No.
CSC-I9187L
Description
ImSt is a conditionally immortalized mouse stomach epithelial cell line isolated from the mouse harboring thermolabile mutation (tsA58) under the control of an interferon (IFN)-γ-inducible H-2Kbpromoter and a temperature-sensitive simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cellsin vitroin medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C) or when cultured without IFN γ over 3 passages, the cells cease to proliferate.
Species
Mus musculus
Source
Stomach
Culture Properties
Adherent
Immortalization Method
Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)
Markers
CK18
Application
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-C9059J B129 Mouse Stomach Epithelial Cells
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
Immunohistochemical techniques were utilized detect keratin 18 and E-cadherin to demonstrate the epithelial nature of cells.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here
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