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Immortalized (Conditionally) Mouse Hepatocytes (ImHep)
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Cat.No.
CSC-I9195L
Description
ImHep is a conditionally immortalized mouse hepatocyte isolated from the mouse harboring thermolabile mutation (tsA58) under the control of an interferon (IFN)-γ-inducible H-2Kbpromoter and a temperature-sensitive simian virus 40 large T antigen. The functional expression of the SV40 large T antigen is induced by culturing the cellsin vitroin medium containing IFN γ at a temperature permissive (33°C). At a non-permissive temperature (37°C-39°C) or when cultured without IFN γ over 3 passages, the cells cease to proliferate. The ImHep cell line is unique in that 1) it is easily transfectable; 2) it is able to persistin vivopost-hepatocllular transplantation at the non-permissive temperature; both of these characteristics make this cell line a valuable tool forin vitrogenetic manipulation, and use in mouse models forex vivogene therapy.
Species
Mus musculus
Source
Liver
Culture Properties
Adherent
Immortalization Method
Isolated from transgenic mouse carrying a temperature-sensitive simian virus 40 tumor antigen (tsA58)
Markers
albumin, AFP, transferrin, LPK, CK8 and CK18
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CSC-C2125 Mouse Hepatocytes
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Western Blot and Immunofluorescence were used to detect hepatocyte markers albumin, AFP, transferrin, LPK, CK8 and CK18;
2) Immunofluorescence were used to verify the presence of SV40 large T antigen
2) Immunofluorescence were used to verify the presence of SV40 large T antigen
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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