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Immortalized Mouse MyD88-/- Hepatic Stellate Cells
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Cat.No.
CSC-I9359L
Description
The Immortalized Mouse Hepatic Stellate Cells were derived from MyD88-/-mouse via enzymatic digestion and Percoll density gradient centrifugation. The primary cells were immortalized via transfection of the pCMV Simian Virus 40 Large T antigen. Myeloid differentiation factor 88 (MyD88) is an adaptor protein downstream of the TLR4 pathway. LPS activation of the Immortalized Mouse MyD88-/-Hepatic Stellate Cells expressing WT TLR4 has shown almost no change in NF-κB activation. The Immortalized Mouse MyD88-/-Hepatic Stellate Cells are useful in studies focusing on liver fibrosis, especially in the role of the inflammatory TLR4 pathway activation in scar tissue formation. In addition, the cells can be used in studies of cellular survival regulation, since TLR4 activation has been linked to apoptosis-resistance.
Species
Mus musculus
Source
Liver
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
pCMV Simian Virus 40 Large T antigen
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Quality Control
1) Genotype analysis;
2) NF-κB Activation Assay;
3) Cytokine ELISA Assays;
4) 3[H]-thymidine Cell Proliferation Assay
2) NF-κB Activation Assay;
3) Cytokine ELISA Assays;
4) 3[H]-thymidine Cell Proliferation Assay
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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