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Immortalized Bcl-x Mouse Embryonic Fibroblasts-SV40T
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Cat.No.
CSC-I9350L
Description
The Immortalized Bcl-x Mouse Embryonic Fibroblasts - SV40T were derived from primary embryonic cells from an 11-d-pregnant Bcl-x heterozygous mouse. The primary cells were immortalized by transfection with pCDNA3 plasmid encoding SV40 large T antigen. The immortalized cells display similar morphological characteristics with the parental cells. The Immortalized Bcl-x Mouse Embryonic Fibroblasts - SV40T is useful in studies in discovering novel signaling pathways, investigating regulatory networks and immune responses particularly related to the Bcl-xL gene.
Species
Mus musculus
Source
Embryo
Culture Properties
Adherent
Morphology
Polygonal
Immortalization Method
Transfection with pCDNA3 plasmid encoding SV40 large T antigen
Applications
For Research Use Only
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Note: Never can cells be kept at -20 °C.
Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
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Quality Control
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
CSC-I9001L
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