A3/KAW
Cat.No.: CSC-C6975J
Species: Homo sapiens (Human)
Source: Ascites
Morphology: Lymphocyte-Like
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Hirose et al. derived the A3/KAW cell line (also referred to as A3/Kawakami) in 1983 from the ascitic fluid of a 68-year-old Japanese woman with gastric malignant lymphoma (diffuse large cell-type non-Hodgkin lymphoma). A3/KAW cells have distinctive immunophenotype of CD4-positive, Ia-negative and EBNA-negative lymphoma cells, on which OKT4 and anti-Leu 3a react. Interestingly, while the original ascitic lymphoma cells displayed surface immunoglobulin positivity, the cultured A3/KAW cells lost expression of definitive B-cell markers. Recent studies suggest that A3/KAW may not express typical B-cell genes such as CD19, CD20, or CD79, raising caution regarding its classification as a diffuse large B-cell lymphoma (DLBCL) model.
A3/KAW cells grow as suspension cells and have a doubling time of 20 hours in RPMI 1640 medium containing 10% fetal bovine serum. A3/KAW cells have a karyotype of 48XY+14q, marker and have 840 known mutations. The cells have many copy number mutations.
IKE Potently Reduces diffuse large B cell lymphoma (DLBCL) Cell Number
The ferroptosis inducer erastin is metabolically labile with low water solubility and potency, limiting in vivo use. IKE, an erastin analog with a carbonyl group (Fig. 1A), forms reversible covalent protein interactions, achieving >100× potency improvement in some cell lines. Isopropoxy substitution enhanced metabolic stability, while the imidazole moiety increased water solubility and ketone stability under acidic conditions.
Zhang's team evaluated DLBCL cell line sensitivity to ferroptosis and established a lymphoma xenograft model. A panel of 18 DLBCL cell lines (LY8, DOHH2, SUDHL5, SUDHL2, SUDHL6, SUDHL10, and SUDHL16 were classified as sensitive cell lines (red). HBL1, SUDHL9, WSU-NHL, SUDHL8, RIVA, and KARPAS422 were classified as moderately resistant cell lines (black). SUDHL7, U937, A3/KAW, LY7, and U2932 were classified as resistant cell lines (blue)) across distinct subtypes showed differential IKE sensitivity: IC₅₀ < 100 nM (sensitive), IC₅₀ > 10 μM (resistant), and intermediate values between 100 nM and 10 μM (Fig. 1B). Co-treatment with ferrostatin-1 (fer-1), a ferroptosis inhibitor, rescued IKE-induced cell death, confirming lipid peroxidation-mediated ferroptosis. Among sensitive lines, SUDHL6 was selected for subcutaneous xenograft generation in 6-week-old male NCG mice.
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