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Cat.No.
CSC-C0469
Description
Established from the pericardial fluid of a 15-year-old man with acute lymphoblastic leukemia (ALL) at relapse (with accompanying thymoma) in 1983; cells were described as: (i) showing NK activity and ADCC (antigen-dependent cell-mediated cytotoxicity), (ii) having their T cell receptor (TCR) genes in germline configuration, and (iii) expressing no TCR proteins (also CD3-, CD16-, CD56+, CD57-)
Species
Human
Source
pericardial fluid
Recommended Medium
90% SuperCult® IMDM + 10% h.i. FBS.
Culture Properties
suspension
Morphology
round to polygonal cells growing singly or in clumps in suspension
Karyotype
Human flat-moded near-tetraploid karyotype with 12% polyploidy - 84-98<4n>XXYY, -6, -9, -15, -19, +6-8mar, der(X)t(X;7)(q25;q21), der(1)dup(1)(q12.2q2?2)t(1;17)(q3?1;q1?2), der(4)t(1;4)(q32.2;q35)x2, der(5)t(X;5)(p1?3;q21)x2, del(6)(p11), der(10;17)(q10;q
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 -, CD3 -, CD4 -, CD5 -, CD6 -, CD7 +, CD8 -, CD13 -, CD19 -, CD25 (+), CD30 +, CD34 -, TCRalpha/beta -, TCRgamma/delta -
Viruses: ELISA: reverse transcrip
Immunology: CD2 -, CD3 -, CD4 -, CD5 -, CD6 -, CD7 +, CD8 -, CD13 -, CD19 -, CD25 (+), CD30 +, CD34 -, TCRalpha/beta -, TCRgamma/delta -
Viruses: ELISA: reverse transcrip
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What are the advantages of ISH?
A:
In situ hybridization (ISH) combines three main advantages, great sensitivity, precise anatomical localization, and the possibility of quantification.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Excellent
Before use, we verify the identity, purity, and viability of the lymphoma cell products by methods such as flow cytometry or microscopy. the quality of Creative Bioarray's products is indeed excellent.
13 July 2022
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