YT

Cat.No.: CSC-C0469

Species: Human

Source: pericardial fluid

Morphology: round to polygonal cells growing singly or in clumps in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0469

Description

Established from the pericardial fluid of a 15-year-old man with acute lymphoblastic leukemia (ALL) at relapse (with accompanying thymoma) in 1983; cells were described as: (i) showing NK activity and ADCC (antigen-dependent cell-mediated cytotoxicity), (ii) having their T cell receptor (TCR) genes in germline configuration, and (iii) expressing no TCR proteins (also CD3-, CD16-, CD56+, CD57-)

Species

Human

Source

pericardial fluid

Recommended Medium

90% SuperCult® IMDM + 10% h.i. FBS.

Culture Properties

suspension

Morphology

round to polygonal cells growing singly or in clumps in suspension

Karyotype

Human flat-moded near-tetraploid karyotype with 12% polyploidy - 84-98<4n>XXYY, -6, -9, -15, -19, +6-8mar, der(X)t(X;7)(q25;q21), der(1)dup(1)(q12.2q2?2)t(1;17)(q3?1;q1?2), der(4)t(1;4)(q32.2;q35)x2, der(5)t(X;5)(p1?3;q21)x2, del(6)(p11), der(10;17)(q10;q

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 -, CD3 -, CD4 -, CD5 -, CD6 -, CD7 +, CD8 -, CD13 -, CD19 -, CD25 (+), CD30 +, CD34 -, TCRalpha/beta -, TCRgamma/delta -
Viruses: ELISA: reverse transcrip

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the advantages of ISH?

In situ hybridization (ISH) combines three main advantages, great sensitivity, precise anatomical localization, and the possibility of quantification.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Excellent

Before use, we verify the identity, purity, and viability of the lymphoma cell products by methods such as flow cytometry or microscopy. the quality of Creative Bioarray's products is indeed excellent.

13 July 2022

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For research use only. Not for any other purpose.