Established from the bone marrow of a 64-year-old man with acute myeloid leukemia (AML M7) at diagnosis in 1988; cells are constitutively cytokine-dependent and responsive to various cytokines
Human flat-moded hypotetraploid karyotype - 72-88<4n>XXYY, +1, -8, -9, -9, -9, -11, -11, -12, -13, -13, -14, -18, -21, -22, +6mar, del(1)(q42)x2, t(2;4)(p16-23;q27-31)x1-2, der(2)t(2;5)(p16-23;q12-14)x1-2, add(5)(q11), del(5)(q13), der(6;13)(p10;q10), i(7
acute myeloid leukemia
round (relatively large) cells growing singly in suspension (ca. 1-2% cells are slightly adherent)
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, CD41 (+), CD42 (+), CD68 +, CD235a (+), HLA-DR (+)
Viruses: ELISA: reverse transcriptase negativ
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x106 cells/ampoule; ship in dry ice; store in liquid nitrogen
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Biomarkers associated with ischemic stroke in diabetes mellitus patients
Authors: Yang S, Zhao J, Chen Y, et al.
The Cardiovascular toxicology. 2016, 16(3): 213-222.