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UT-7

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Cat.No.
CSC-C0294
Description
Established from the bone marrow of a 64-year-old man with acute myeloid leukemia (AML M7) at diagnosis in 1988; cells are constitutively cytokine-dependent and responsive to various cytokines
Species
Human
Source
acute myeloid leukemia
Recommended Medium
Culture Properties
suspension
Morphology
round (relatively large) cells growing singly in suspension (ca. 1-2% cells are slightly adherent)
Karyotype
Human flat-moded hypotetraploid karyotype - 72-88<4n>XXYY, +1, -8, -9, -9, -9, -11, -11, -12, -13, -13, -14, -18, -21, -22, +6mar, del(1)(q42)x2, t(2;4)(p16-23;q27-31)x1-2, der(2)t(2;5)(p16-23;q12-14)x1-2, add(5)(q11), del(5)(q13), der(6;13)(p10;q10), i(7
Reference
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD4 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, CD41 (+), CD42 (+), CD68 +, CD235a (+), HLA-DR (+)
Viruses: ELISA: reverse transcriptase negativ
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x106 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
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Q:
Why wash cell culture with PBS?
A:

PBS is often used for washing due to being isotonic and non-toxic to cells and tissues and thus allows for cells to be rinsed of unwanted media without potentially lysing them.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Well-characterized

The cells were well characterized and matched the description provided.

27 May 2022


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