UT-7

Cat.No.: CSC-C0294

Species: Homo sapiens (Human)

Source: Bone Marrow

Morphology: round (relatively large) cells growing singly in suspension

Culture Properties: Suspension

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Cat.No.

CSC-C0294

Description

established from the bone marrow of a 64-year-old man with acute myeloid leukemia (AML M7); cells are constitutively cytokine-dependent and responsive to various cytokines

Species

Homo sapiens (Human)

Source

Bone Marrow

Recommended Medium

RPMI 1640 w/o Hepes + 10% FBS +5-7u/ml Epo

Culture Properties

Suspension

Morphology

round (relatively large) cells growing singly in suspension

STR DNA Profile

Amelogenin: X,Y D5S818: 12 D13S317: 8 D7S820: 8 D16S539: 10,12 vWA: 14,18 TH01: 6,9 TPOX: 10 CSF1PO: 12 D19S433: 14,14.2 D21S11: 31.2 D18S51: 14,17 D6S1043: 10,19 D3S1358: 16 Penta D: 9,11 D2S441: 10,11 D8S1179: 11,15 Penta E: 16,17 D12S391: 18,19 D2S1338: 18,23 FGA: 23

Karyotype

human flat-moded hypotetraploid karyotype - 72-88<4n>XXYY, +1, -8, -9, -9, -9, -11, -11, -12, -13, -13, -14, -18, -21, -22, +6mar, del(1)(q42)x2, t(2;4)(p16-23;q27-31)x1-2, der(2)t(2;5)(p16-23;q12-14)x1-2, add(5)(q11), del(5)(q13), der(6;13)(p10;q10), i(7q)x1-2, add(8)(q24)x1-3, del(9)(q22), add(12)(p12-13), der(13)t(13;13)(p11;q14), add(14)(p11), add(15)(p11), add(16)(p1?), add(17)(p11), add(19)(p13)x2, add(19)(q13), i(21q)x1-2 - extensive subclonal variation present - resembles published karyotype

Reference

Publication

Disease

Acute Megakaryoblastic Leukemia

Quality Control

Mycoplasma: negative in PCR assays
Immunology: CD3 -, CD4 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, CD41 (+), CD42 (+), CD68 +, CD235a (+), HLA-DR (+)
Viruses: ELISA: reverse transcriptase negative

Storage and Shipping

ship in dry ice; store in liquid nitrogen

Synonyms

UT7

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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