U-2973

Specification
Recommended Products

Cat.No.

CSC-C0627

Description

Established in 2006 from the peripheral blood of a 42-year-old Caucasian man with mature B-cell leukemia; described to carry t(14;18)(q32;q21) with IGH@-BCL2 and biallelic MYC rearrangement with non-IG partner

Species

Human

Source

B cell lymphoma

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS

Culture Properties

suspension

Morphology

round cells growing singly in suspension

Karyotype

Human hyperdiploid karyotype with 4% polyploidy; 47(44-48)XY, +12, del(6)(q24.2q26), t(8;12)(q24;p12)x2, t(14;18)(q32;q21), der(20)t(18;20;q21;q13); t(14;18) effects rearrangement of IGH@ and BCL2; t(8;12) effects non-IG, MYC rearrangement; resembles publ

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD138 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 9 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.