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U-2973

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Cat.No.
CSC-C0627
Description
Established in 2006 from the peripheral blood of a 42-year-old Caucasian man with mature B-cell leukemia; described to carry t(14;18)(q32;q21) with IGH@-BCL2 and biallelic MYC rearrangement with non-IG partner
Organism
Human
Karyotype
Human hyperdiploid karyotype with 4% polyploidy; 47(44-48)XY, +12, del(6)(q24.2q26), t(8;12)(q24;p12)x2, t(14;18)(q32;q21), der(20)t(18;20;q21;q13); t(14;18) effects rearrangement of IGH@ and BCL2; t(8;12) effects non-IG, MYC rearrangement; resembles publ
Source
B cell lymphoma
Culture Properties
suspension
Morphology
round cells growing singly in suspension
Recommended Medium
80-90% RPMI-1640 + 10-20% h.i. FBS
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD138 +, HLA-DR +, sm/cyIgG +, sm/cyIgM -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 9 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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