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TMM
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Cat.No.
CSC-C0246
Description
Established from the peripheral blood of a 62-year-old man with Philadelphia chromosome negative chronic myeloid leukemia (CML) in blast crisis in 1985; cells were described to be Ph-negative, BCR-ABL1-negative; cells appear to be rather a "normal" B-lymphoblastoid cell line (B-LCL)
Species
Human
Source
B lymphoblastoid cells (from patient with chronic myeloid leukemia in blast crisis)
Recommended Medium
90%SuperCult® IMDM + 10% h.i. FBS
Culture Properties
suspension
Morphology
polygonal cells, single or in clusters in suspension
Karyotype
Human near-diploid karyotype with 8% polyploidy - 46(42-46)<2n>XY - small ring present in 6% cells - no consistent numerical or structural rearrangement - resembles published karyotype
Quality Control
Mycoplasma: negative in DAPI, microbiological culture assays
Immunology: CD3 -, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD33 -, CD37 +, HLA-DR +, anti-EBNA2 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda -
Viruses: ELISA: reverse transcripta
Immunology: CD3 -, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD33 -, CD37 +, HLA-DR +, anti-EBNA2 +, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda -
Viruses: ELISA: reverse transcripta
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5-10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
Why does media turn yellow?
A:
A yellow hue to the medium indicates the pH is too low (acidic), a purple hue shows the pH is too high (alkaline) whereas an orangey-red hue indicates the medium is of the correct physiological pH.
Average Rating: 5.0 | 1 Scientist has reviewed this product
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19 Nov 2022
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