Sertoli cells dissected from normal testis from 17-day-old Wistar rat and immortalized with SV40 large-T antigen; cells were described as displaying morphological and functional features of Sertoli cells (phagocytosis, cisplatin sensitivity, transferrin secretion)
Rat flat-moded hypotetraploid karyotype - 70-78<4n>XXYY/XXY - supernumerary r/acf present in most cells
epitheloid cells growing in monolayers
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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