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SER-W3
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Cat.No.
CSC-C1387
Description
Sertoli cells dissected from normal testis from 17-day-old Wistar rat and immortalized with SV40 large-T antigen; cells were described as displaying morphological and functional features of Sertoli cells (phagocytosis, cisplatin sensitivity, transferrin secretion)
Species
Rat
Source
rat Sertoli cells
Culture Properties
monolayer
Morphology
epitheloid cells growing in monolayers
Karyotype
Rat flat-moded hypotetraploid karyotype - 70-78<4n>XXYY/XXY - supernumerary r/acf present in most cells
Quality Control
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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