Established from the sarcoma of the thyroid gland from a 47-year-old woman in 1985
Human flat-moded hexaploid/octaploid karyotype with 14% polyploidy - 156(140-188)<6n>XXXX, -X, -X - multiple copies of: i(1p), i(1q), i(3p), i(6p), i(9q), add(5)(p15), add(16)(q23), i(mar) - 1-3 dmin/rings in 40% cells - isochromosome formation previously
epitheloid cells growing adherently as monolayer
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 (+), cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP +, neurofilament -, th
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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