RI-1
Cat.No.: CSC-C0590
Species: Homo sapiens (Human)
Source: Blood; Peripheral Blood
Morphology: large round cells growing in suspension
Culture Properties: suspension
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Immunology: CD3 -, CD10 -, CD13 -, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMR
RI-1 is a human B-cell non-Hodgkin lymphoma (B-NHL) cell line, originally established from a patient with diffuse large B-cell lymphoma (DLBCL). This cell line is typically used for in vitro research and is known for its stable growth in suspension culture. Phenotypically, RI-1 cells are positive for B-cell markers such as CD19, CD20, CD22, and surface immunoglobulin, consistent with mature B-cell lineage. At the molecular level, RI-1 cells exhibit alterations in several signaling pathways commonly implicated in B-cell lymphomas, including B-cell receptor (BCR) signaling, NF-κB activation, and apoptosis regulation. Genetically, RI-1 cells possess genetic lesions often associated with B-cell lymphomas, providing a relevant model for the study of B-cell lymphoma pathogenesis and treatment.
RI-1 cell line is commonly used for studying B-cell lymphoma pathogenesis and signal transduction, as well as for preclinical testing of immune-targeted therapies. This cell line is also valuable for the screening and evaluation of potential therapeutic agents targeting B-cell surface antigens, BCR signaling components, and apoptosis regulators, supporting lymphoma research and drug discovery efforts.
Determination of the Minimum Cell-to-Cell Adhesion Time Using Optical Tweezers in Leukemia and Lymphoma Research
Single-cell adhesion assays study attachment and detachment. Optical tweezers (OTs) are great for this because they're gentle and don't need labels. OTs can measure tiny forces and study how long cells need to touch to stick together.
Duś-Szachniewicz et al. made a step-by-step guide to use OTs with low laser power to study how leukemia-lymphoma cells stick to other cells. Their method is very precise and can spot small changes in cell adhesion, as shown in Figure 1. They found big differences between two cell lines, Ri-1 and Toledo. Ri-1 cells stuck to other cells in 5-20 seconds, while Toledo cells took 60-240 seconds. The average time for Ri-1 was 12.83 ± 4.94 seconds, much faster than Toledo's 141 ± 49.69 seconds. Figures 1C and D show how many cells stuck over time. They tested both early (passage 3) and later (passage 6) cells and found no big differences. Their results match a previous study and suggest that different cell lines have different surface properties or adhesion mechanisms. Optical tweezers are way better than traditional methods, which can only measure adhesion times of 30-60 minutes and often miss important details. Single-cell studies help us understand cell differences and how tissues and organs work.
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