Established from tumor material of a previously subcutaneously passaged rapidly growing, hormone independent, anaplastic and metastatic variant of the rat Dunning R 3327 AT prostate tumor in Copenhagen rats; cells were described to be androgen insensitive and to have a high metastatic potential with dissemination to lymph nodes and lungs in rats
polymorphic cells growing adherently in monolayers
Mycoplasma: negative in microbiological culture, PCR assays
Viruses: PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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