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OMEGA-E
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Cat.No.
CSC-C1083
Description
Derived from NIH-3T3 cells; high titer ecotropic helper-free packaging cell line; generated with separate gagpol and ecotropic env expression constructs with minimal sequence overlap and decreased sequence homology achieved by "codon wobbling"; prior to use in nude mice, cells need to be subcloned; classified as risk category S1 according to the German Central Commission for Biological Safety (ZKBS)
Species
Mouse
Source
NIH-3T3 (Swiss mouse embryo) derivative-retroviral packaging cell line
Recommended Medium
90% DMEM + 10% NCS
Culture Properties
monolayer
Morphology
cells grow as adherent fibroblastic monolayers
Karyotype
Murine hypertriploid karyotype - 71(63-73)<3n> - 1-2 dm (24% cells)
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Cytogenetics: murine hypertriploid karyotype - 71(63-73) - 1-2 dm (24% cells)
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Cytogenetics: murine hypertriploid karyotype - 71(63-73) - 1-2 dm (24% cells)
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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