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NUC-1
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Cat.No.
CSC-C1384
Description
Established from an adult female C3H/Law mouse; tumorigenic, invasive in vitro immortalized urine bladder cell line, aneuploid (chromosome number 70-75)
Species
Mouse
Source
urinary bladder
Recommended Medium
45% Hams F10 + 45% DMEM + 10% h.i. FBS + 5 μg/ml Human insulin + 5 μg/ml Human transferrin + 50 nM hydrocortisone + 5 ng/ml selenite + 10 μM HEPES
Morphology
spindle cell-like, disorderly arranged cells, growing in multilayers
Karyotype
Hypotetraploid flat-moded murine karyotype with limited centric fusion, dm present in 25% cells - 70(66-75)<4n>
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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