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NU-DHL-1

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Cat.No.
CSC-C0588
Description
Established from the left inguinal lymph node of a 73-year-old Caucasian man with B-cell Non-Hodgkin lymphoma (B-NHL, diffuse large cell lymphoma, non-cleaved cell type) in 1982; assigned to GCB-like lymphoma subtype (germinal center B-cell)
Organism
Human
Karyotype
Human hyperdiploid karyotype with 4% polyploidy - 51(46-53)<2n>XY, +5, +8, +9, +12, +12, t(3;8)(p25;q24), i(5p), dup(7)(q21.3q31.1), der(8)t(3;8)(p25;q24), i(9p), del(12)(q11), i(12p), der(14)t(14;18)(q32;q21)x1-3 - sideline with dup(2)(p2?1p2?4) - carrie
Source
B cell lymphoma
Culture Properties
suspension
Morphology
single, round to polymorph cells growing in suspension
Recommended Medium
80-90% RPMI-1640 + 10-20% h.i. FBS
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, fm/cykappa -, sm/cylambda +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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