Established ca. 1986 from the cholangio-hepatocellular tumor induced in vivo by treatment of a male Wistar AF-Han rat with a single injection of N-methyl-N-nitrosourea and oral administration of phenobarbital
epitheloid cells growing adherently in monolayers
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +
Viruses: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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