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Cat.No.

CSC-C6235X

Description

Established from peripheral blood lymphocytes of a 27-year-old Caucasian woman by Epstein-Barr virus (EBV) transformation in 1990; suitable normal control cell line for toxicity or radiosensitivity assays

Species

Homo sapiens (Human)

Source

Blood; Peripheral Blood

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, polymorph cells or small clumps in suspension

Karyotype

Human near diploid karyotype with hypodiploid sideline - 45/46(42-46)<2n>XX/X, -X - no structural rearrangements detected

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 (+), cyCD79a (+), CD80 +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, smkappa -, cykappa +, sm/cylambda

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.