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NAMALWA.PNT

  • Specification
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Cat.No.
CSC-C0230
Description
The NAMALWA.PNT cell line is a subclone of the cell line NAMALWA. Established from the tumor mass of an African child with Burkitt lymphoma; this variant subline from the original NAMALWA was described to be characterized by differential expression of immunoglobulin and MYC genes and of some cell surface antigens (see reference); cells were reported to harbor a retrovirus of simian origin, SMRV-H confirmed as human with IEF of AST, MDH, NP.
Species
Homo sapiens, human
Source
Burkitt lymphoma
Recommended Medium
Culture Properties
Suspension
Morphology
lymphoblastoid, single cells with irregular forms or small clusters, in suspension
STR DNA Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 7,9,3
TPOX: 6,11
vWA: 16

Karyotype
Presence of NAMALWA marker chromosomes confirmed
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD37 (+), HLA-DR +, sm/cyIgM +, sm/cyIgG -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

  • Q & A
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Q:
What are the cell culture techniques?
A:

There are two cell culture techniques to grow cells in culture, monolayers on an artificial substrate (i.e., adherent culture) or free-floating in a culture medium (suspension culture).

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Average Rating: 4.0    |    1 Scientist has reviewed this product

Highly recommended

These tumor cell products were even better than those described in my experiment. Highly recommended!

03 May 2023


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