Description
The NAMALWA.PNT cell line is a subclone of the cell line NAMALWA. Established from the tumor mass of an African child with Burkitt lymphoma; this variant subline from the original NAMALWA was described to be characterized by differential expression of immunoglobulin and MYC genes and of some cell surface antigens (see reference); cells were reported to harbor a retrovirus of simian origin, SMRV-H confirmed as human with IEF of AST, MDH, NP.
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 7,9,3
TPOX: 6,11
vWA: 16
Karyotype
Presence of NAMALWA marker chromosomes confirmed
Culture Properties
Suspension
Morphology
lymphoblastoid, single cells with irregular forms or small clusters, in suspension
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD37 (+), HLA-DR +, sm/cyIgM +, sm/cyIgG -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
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USA: 1-631-626-9181
Europe: 44-207-097-1828
