The NAMALWA.PNT cell line is a subclone of the cell line NAMALWA. Established from the tumor mass of an African child with Burkitt lymphoma; this variant subline from the original NAMALWA was described to be characterized by differential expression of immunoglobulin and MYC genes and of some cell surface antigens (see reference); cells were reported to harbor a retrovirus of simian origin, SMRV-H confirmed as human with IEF of AST, MDH, NP.
Homo sapiens, human
STR DNA Profile
Presence of NAMALWA marker chromosomes confirmed
lymphoblastoid, single cells with irregular forms or small clusters, in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD37 (+), HLA-DR +, sm/cyIgM +, sm/cyIgG -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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