6-thioguanine resistant mutant of cell line N4 that was established from a mouse of strain A/J with neuroblastoma C1300; cells were described to form increased levels of cAMP in response to prostaglandin E1 which is inhibited by opiate receptors; cells are aneuploid (modal chromosome number of 86)
flat cell body, slightly adherent, occasionally with short processes
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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