MEC-1

Specification
Recommended Products

Cat.No.

CSC-C0508

Description

Established in 1993 from the peripheral blood of a 61-year-old Caucasian man with chronic B cell leukemia (B-CLL in prolymphocytoid transformation to B-PLL); serial sister cell line of MEC-2

Species

Human

Source

chronic B cell leukemia

Recommended Medium

90% SuperCult® IMDM + 10% h.i. FBS.

Culture Properties

suspension

Morphology

round to polymorphic cells growing in suspension, singly or partly in small aggregates, a few cells are slightly adherent

Karyotype

Human near-diploid karyotype with 10% polyploidy - 46(44-47)<2n>XY, -2, +7, -12, +1-2mar, t(1;6)(q22-23;p21), add(7)(q11), der(10)(10pter->q22::?::2q11->qter), del(17)(p11) - small acf/mar present in most cells - resembles published karyotype

Quality Control

Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 +

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 9 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1450Y

CS-001Y

For research use only. Not for any other purpose.