a primary liver carcinoma was induced by dimethyl-aminoazobenzene (DAB) in a Wistar-Commentry rat; the hepatoma was transferred to a syngeneic rat and a cell line was established and subcloned in 1966
Rat flat-moded near-triploid karyotype - 58-67 - large submetacentric marker present in most cells
epithelial-like cells growing as monolayers
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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