CAL-39

Cat.No.: CSC-C0316

Species: Homo sapiens (Human)

Source: Vulva

Morphology: adherent, epithelial-like cells growing as monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C0316

Description

Established from the primary lesion resected from a 76-year-old woman with bulky, moderately differentiated squamous cell carcinoma of the vulva in 1984; cells were reported to express an elevated number of high affinity EGF receptors, to carry gene amplification at 11q13 and to be tumorigenic in nude mice

Species

Homo sapiens (Human)

Source

Vulva

Recommended Medium

80% DMEM + 20% h.i.FBS + 0.5 nM hydrocortisone + 1 μg/100 ml EGF

Culture Properties

monolayer

Morphology

adherent, epithelial-like cells growing as monolayer

Karyotype

Human flat-moded hypotriploid karyotype with 27% polyploidy - 59(54-60)<3n>XX, -X, +1, +1, +2, +3, +9, +12, +12, +16, +20, +22, +4mar, del(1)(q22), del(3)(p14), add(4)(p16), del(7)(q32), add(9)(p22), del(12)(q15), add(12)(q24), add(18)(q23) - additional 1

Disease

Vulvar Squamous Cell Carcinoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.75-1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.