Description
Established from the primary lesion resected from a 76-year-old woman with bulky, moderately differentiated squamous cell carcinoma of the vulva in 1984; cells were reported to express an elevated number of high affinity EGF receptors, to carry gene amplification at 11q13 and to be tumorigenic in nude mice
Karyotype
Human flat-moded hypotriploid karyotype with 27% polyploidy - 59(54-60)<3n>XX, -X, +1, +1, +2, +3, +9, +12, +12, +16, +20, +22, +4mar, del(1)(q22), del(3)(p14), add(4)(p16), del(7)(q32), add(9)(p22), del(12)(q15), add(12)(q24), add(18)(q23) - additional 1
Source
vulva squamous cell carcinoma
Culture Properties
monolayer
Morphology
adherent, epithelial-like cells growing as monolayer
Recommended Medium
80%
DMEM + 20%
h.i.FBS + 0.5 nM hydrocortisone + 1 μg/100 ml EGF
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, desmin -, endothel -, GFAP -, neurofilament -, vimentin -
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 0.75-1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no.
If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
USA: 1-631-626-9181
Europe: 44-207-097-1828
