Established from the primary tumor of a 3-year-old girl with neuroblastoma (clinical Stage IV)
adherent flat cells with processes accompanied by loosely adherent round cells
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament +, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -
Storage and Shipping
Frozen with 35% RPMI-1640, 35% MEM (with Earles salts), 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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