This Adriamycin-resistant cell line has been developed by exposure of the parent L929 murine fibroblast cell line to increasing concentrations of doxorubicin in-vitro. L929/A cells can be used in the development of novel anti-cancer treatments. Resistance can be circumvented by modulating agents such as verapamil and quinine. The parent cell line L929 was derived from normal subcutaneous areolar adipose tissue.
RPMI 1640 + 10% Foetal Bovine Serum (FBS) + 2mM L-Glutamine + 0.086uM Doxorubicin hydrochloride.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
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