K562/AZQR

Cat.No.: CSC-C9504J

Species: Homo sapiens (Human)

Source: Pleural Effusion

Morphology: Lymphoblastoid

Culture Properties: Suspension

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Cat.No.

CSC-C9504J

Description

The drug resistant cell line K562 AZQ has been developed from the parent K562 cell line by treatment with AZQ C215-bis (carboethoxyamino)-3, 6-diazitinyl 1;4 (benzoquinone). The cells have been shown to contain decreased levels of glutathione and superoxoid dismutase. They are cross-resistant to adriamycin, mitzolamide, MMNG and mitmycin. Cells should be challenged with AZQ at every 4th passage at a minimum density of 100,000 cells/ml. It is recommended to cultivate the cells without drug on resuscitation.

Species

Homo sapiens (Human)

Source

Pleural Effusion

Recommended Medium

RPMI 1640 + 10% Fetal Bovine Serum (FBS) + 2mM Glutamine. Challenge with 1 mM AZQ every 4th passage.

Culture Properties

Suspension

Morphology

Lymphoblastoid

Disease

Chronic Myeloid Leukemia

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.

Synonyms

K562 AZQR; K562-AZQR; K562AZQR; AZQR

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.