Established from the bone marrow of a 20-year-old woman with Hodgkin lymphoma (nodular sclerosis; stage IVB, pre-terminal stage)
Human flat-moded hypotriploid karyotype with 16% polyploidy - 59-64<3n>XX, -X, -1, +2, -6, +7, -8, -9, -10, -11, -13, -14, -16, -17, -18, +22, +5-6mar, der(1;5)(q10;p10)x1-2, der(2;12)(q10;q10), der(2)t(2;?8)(q3?7;q2?1)x1-2, ins(5;?)(p11;?), add(7)(p1?),
round cells growing singly in suspension
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 +, CD3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD21 -, CD25 +, CD30 +, CD34 -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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A novel enediyne-integrated antibody-drug conjugate shows promising antitumor efficacyagainst CD30+ lymphomas.
Authors: Rong Wang, Liang Li, Shenghua Zhang, Yi Li, Xiaofei Wang, Qingfang Miao, Yongsu Zhen
Molecular Oncology, 2018, 12(3): 339-355.