KE-37

Cat.No.: CSC-C0218

Species: Homo sapiens (Human)

Morphology: single, round cells in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0218

Description

Established from a 27-year-old man with acute lymphoblastic leukemia (ALL) in 1979

Species

Homo sapiens (Human)

Recommended Medium

90-92.5% RPMI-1640 + 7.5-10% h.i. FBS

Culture Properties

suspension

Morphology

single, round cells in suspension

Karyotype

Human flat-moded hypotetraploid karyotype - 91(86-92)<4n>XXYY, +8, -14, -14, t(7;12)(q32-33;p12-13)x2, der(8)t(8;14)(q24;q11)x4, der(14)t(8;14)(q24;q11)x2 - resembles published karyotype - carries 4 copies of der(8)t(8;14) associated with T-ALL - normal c

Disease

Adult T Acute Lymphoblastic Leukemia

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD2 +, cyCD3 +, smCD3 -, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/b

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 8 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

KE 37; KE37

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.